Detecting structural damage in operational conditions still encounters some difficulties, especially in early-stage, as environmental varieties impose challenges in real engineering applications and may require large computational efforts in the structural health monitoring and potential maintenance. Unlike conventional strategies employing frequency response function or response data, a damage detection methodology is addressed in this study by employing transmissibility functions that retains a strong interrelation with structural damage or deterioration, in order to avoid the measurement of excitation, together with principal component analysis that leads to reduction in computational costs. In this procedure, transmissibility is extracted from the structural responses and main features are selected by principal component analysis for less computational costs. Then, via distance measures damage indicators are constructed for both intact and damaged states, and finally a numerical simulation with a clamped-clamped beam and a four-story benchmark are adopted to verify the applicability of the proposed procedure. The results demonstrate a good performance in structural damage detection.