Novel biocomposites made of an acrylated epoxidized hemp oil based bioresin reinforced with random hemp fiber mat were manufactured by the vacuum infusion technique. Mechanical properties (tensile, flexural, Charpy impact and interlaminar shear), dynamic mechanical properties (glass transition temperature, storage modulus and crosslink density) and moisture absorption properties (saturation moisture level and diffusion coefficient) were investigated and compared with samples manufactured under the same conditions but using a commercial synthetic vinylester resin as the polymeric matrix. Results showed that the 100% biocomposites mechanical performance is comparable to that of the hybrid composites made with the synthetic resin. Moisture absorption tests showed that acrylated epoxidized hemp oil based samples displayed both higher diffusion coefficient and saturation moisture content; however, fiber reinforcement was the dominant transfer mechanism. Vinyl ester based samples were found to have higher storage modulus, glass transition temperature and crosslink density than acrylated epoxidized hemp oil samples.