The main research objective of this study was to investigate the effect of the impregnation of Twaron® fabric with various shear thickening fluids (STFs) on the stab resistance at quasi-static conditions—being the main parameter characterizing the future application of the impregnated textiles in the scope of the new functionality of the elaborated protection of the multi-layer system. It was found that the STF/Twaron® fabric composites required significantly higher loading than the untreated fabric to achieve spike penetration. In the composite fabric tests, the spike did not fully puncture the material.