In this paper, the free vibration of an orthotropic beam undergoing finite strain are studied. The second Piola-Kirchhoff stress tensor and Green-Lagrange strain tensor according to finite strain assumption were used to obtain Euler-Bernoulli beam governing equations. The Galerkin method and Generalized Differential Quadrature method were employed for solving the governing equations and boundary condition. The effect of beam thickness and different boundary conditions were considered in finite strain formulation of the beam equations. Natural frequencies of different composite materials are obtained and compared. The results revealed that by increasing the beams thickness, the difference between maximum vibration amplitude increased between von Karman and finite strain formulations. Also, in a beam with simply- simply supports, differences between linear and non linear mode shapes was remarkable.