Evaluation of recombinase polymerase amplification assays for targeted detection of bovine respiratory disease bacterial pathogens and antimicrobial-resistance genes in feedlot calves

Tara Funk, Lianne McLeod, Rahat Zaheer, Curtis Claassen, Christina Yevtushenko, Cheyenne Conrad, Jennifer Abi Younes, Morgan Lehmann, Sheryl Gow, Bruce Wobeser, Simon J. G, Cheryl Waldner, Tim McAllister

Journal of Veterinary Diagnostic Investigation

Published online on March 05, 2026

Abstract

Journal of Veterinary Diagnostic Investigation, Volume 38, Issue 3, Page 458-472, May 2026.
Antimicrobial resistance (AMR) challenges the effective treatment of bovine respiratory disease (BRD). We evaluated the performance of a recombinase polymerase amplification (RPA) assay, a rapid, isothermal nucleic-acid amplification method, compared with ...