Two novel antibacterial materials were prepared by immobilizing antibacterial peptides protamine sulfate and polymyxin sulfate on polyethylene terephthalate (PET) non-woven fabrics in this study. The fabrics were surface modified by a chemical procedure to create carboxyl groups followed by grafting coupling agent and immobilization of peptide. Scanning electron microscopy images showed that there were no changes on the surface of the fabrics after treatment. X-ray photoelectron spectroscopy confirmed that protamine and polymyxin were successfully grafted on the surface of the PET fabrics. Antibacterial testing using the liquid droplet method showed that fabrics treated with both peptides had excellent antibacterial activity against Escherichia coli and Staphylococcus aureus.