Value-based conflicts are prone to escalation and rather insensitive to standard conflict resolution techniques. To understand why this is the case, we assessed self-regulatory and cardiovascular (CV) responses to test how people cope with conflict, depending on whether values versus resources are at stake. Our results show that a value conflict induces a CV threat profile and raises a prevention focus. Conversely, a resource conflict induces a CV challenge profile and decreases prevention focus. These results suggest that value conflicts are linked to more prevention-focused motivational profiles than resource conflicts. This knowledge can foster the development of specific strategies to facilitate resolution of value conflicts.