Human hairs are considered as major waste from barbershops. It contains an important protein called keratin. Keratin from human hair exhibits biocompatibility, wound-healing property, biodegradability, and antibacterial property. Silver and gold nanoparticles are commonly used in wound-healing applications. These nanoparticles possess excellent functional properties like antibacterial, wound healing and biocompatibility and applicable in biomedical field. In this study, silver and gold nanoparticles were used along with human hair keratin for the production of antibacterial materials. The functional properties of these two materials are synergized for wound-healing applications. Silver and gold nanoparticles were synthesized by chemical reduction method. The synthesized nanoparticles were characterized using UV–Visible, particle size, and zeta potential analyses. The prepared materials were characterized by scanning electron microscopy, Fourier transform infrared, and energy-dispersive X-ray spectroscopic analyses and they were measured for the physical properties such as air permeability, water contact angle, moisture content, and water absorbency. Furthermore, the antibacterial activities were evaluated against burn wound bacteria. Superior antibacterial activities and adequate physical properties found in keratin/nanoparticles immobilized cotton samples.