The dynamic sweat transfer tester for analyzing the sweat transfer behavior of multi-weave structure fabrics (single fabric contains different weave structures) has been designed and developed. This instrument has been developed to evaluate the sweat transfer rate in 16 different directions and 48 different regions for a test specimen of 7 cm diameter. The concept of the sweat measurement is to measure the sweat transport time required to reach the unit area of the fabric. The instrument works under the principle of electrical conductivity, that is, wetted cotton fabric acts as an electrical conductor between the power connected (5 V) copper pins and ground connected copper pins. The unique feature of this instrument is that the sweat transfer rate can be measured in fabrics which have irregular spreading behavior (single fabric that has different weave structures) at one step. Twelve different woven fabrics have been analyzed for the sweat transfer behavior using the instrument, and the results were well correlated (R² = 0.925) with manual test method.